Borrelia IgM ELISA
|Method:||Sandwich ELISA, HRP/TMB, 12x8-well strips|
|Sample type:||Serum, plasma, CSF|
|Controls:||3 vials (negative, cut-off, positive)|
|Sample size:||100 µl (diluted sample) / well|
|Incubation time:||1 h / 30 min / 15 min|
Intra-assay (n=12) ≤ 7%, Inter-assay (n=17) ≤ 8%
Principle of the assay:
Manual ELISAs - easily adaptable for automation!
INSTRUCTIONS FOR USE
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Lyme borreliosis is a bacterial infection caused by the spirochaete Borrelia burgdorferi sensu stricto, afzellii, or garinii, and is characterised by a variety of clinical symptoms. It can be divided into 3 stages:
Stage 1, early dermatitis, Clinical: erythema migrans.
Stage 2, early disseminated infection, Clinical: lymphocytic meningoradiculitis (Bannwarth's syndrome), neuroborreliosis.
Stage 3, late disseminated infection, Clinical: chronic progressive encephalomyelitis, acrodermatitis chronica atrophicans (ACA), chronic arthritis.
To improve the diagnostic specifity, the Biomedica microwell strips are coated with the following recombinant antigens:
· p21 = OspC (outer surface protein C): B. afzellii (pKo)
· p21 = OspC (outer surface protein C): B. garinii (20047)
· p41/I = (inner part of flagellin): B. bavariensis (pBi)
· VIsE: fusion proteins of different Borrelia genospecies
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