Cardiac Safety Biomarkers
Cardiovascular toxicity (CV) plays a significant role in the failure of drug development. The use of blood-based biomarkers provides a way to detect CV toxicity during the preclinical stage, enabling the prioritization of compounds that show a lower risk inducing such adverse effects.
Cardiac Safety Biomarkers
The natriuretic peptides NT-proANP and NT-proBNP are cardiac hormones that are secreted when the heart muscle stretches. These peptides can be measured in blood samples and are well-known preclinical safety biomarkers used throughout drug development e.g NT-proBNP and NT-proANP – markers of drug-induced hypertrophy in rats (1-3).
BIOMEDICA provides robust ELISA assays for the accurate measurement of the these cardiac hormones for both human and rodent samples.
BIOMEDICA´s NT-proANP ELISA kit (BI-20892) has been independently validated in preclinical toxicology testing in rats:
In brief, the assay was evaluated by the Predictive Safety Testing Consortium, Cardiac Hypertrophy Working Group (PSTC—CHWG) in a cross-laboratory (5 laboratories) analytical assessment for use with rat samples. In summary, the assay has proven to be robust and technically adequate for the detection of NT-proANP serum levels in SD rats.
NT-proANP ELISA assay (cat. no. BI-20892)
- 10 µl / well, serum or plasma
- widely cited as cardiovascular safety biomarker in rats
- for use in human and non-human samples (high cross-reactivity between species)
Rat NT-pro BNP ELISA assay (cat. no. BI-1204R) NEW
contact us for your special evaluation discount info@bmgrp.com
- 10 µl / well, serum or plasma
- kit control included
- sample values provided
Example of a Biomedica ELISA kit
PRODUCT INFORMATION
- Product code: BI-20892
- Method: ELISA
- Time to result: 3.5 hours
- Sample types: Serum, plasma, urine, cell culture supernatant (human, rat, mouse samples)
- Sample volume: 10 µl/well
- Sensitivity LOD: 0.05 nmol/l (= 0.64 ng/ml)
- Standard curve range: 0 – 10 nmol/l (= 0 – 127 ng/ml)
- Specificity: Endogenous and recombinant human NT-proANP (equivalent to proANP 1-98). Very high sequence homology between human and rodent (rat, mouse, and other species e.g. rabbit).
- NT-proANP ELISA assay is suitable for rat and mouse samples
- Instructions for use: click here
- Product code: BI-1204R
- Method: ELISA
- Time to result: 3.5 hours
- Sample types: rat serum and plasma
- Sample volume: 10 µl/well
- Sensitivity LOD: 21 pg/ml
- Standard curve range: 0 – 3200 pg/ml
- Specificity: Endogenous and recombinant rat NT-proBNP
- Instructions for use click here
Also available:
NT-proBNP human ELISA (cat. no. SK-1204), CE-marked, kit includes 2 controls
CARDIAC SAFETY BIOMARKER ASSAYS in PRECLINICAL TOXICOLOGY TESTING
References/Citations/Related Literature
All Citations on NT-proANP as a cardiovascular safety biomarker in rat and mouse samples , click for all references
- Cross-laboratory analytical validation of the cardiac biomarker NT-proANP in rat. Vinken P, Reagan WJ, Rodriguez LA, Buck WR, Lai-Zhang J, Goeminne N, Barbacci G, Liu R, King NM, Engle SK, Colton H.J Pharmacol Toxicol Methods. 2016; 77:58-65. doi: 10.1016/j.vascn.2015.10.002. PMID: 26516096.
- Cardiac Hypertrophy Working Group of the Predictive Safety Testing Consortium. Serum Natriuretic Peptides as Differential Biomarkers Allowing for the Distinction between Physiologic and Pathologic Left Ventricular Hypertrophy. Dunn ME et al., Toxicol Pathol. 2017; 45(2):344-352.
- Natriuretic Peptides as Cardiovascular Safety Biomarkers in Rats: Comparison With Blood Pressure, Heart Rate, and Heart Weight. Engle SK, Watson DE.Toxicol Sci. 2016; 149(2):458-72. doi: 10.1093/toxsci/kfv240. Epub 2015 Nov 25. PMID: 26609138.