Accurate Detection of NT-proBNP in Rats - A Cardiac Safety Biomarker

Study Reveals Reliable Measurement of NT-proBNP in Rats Using Biomedica ELISA Assay in Cardiac Overload Model

We are pleased to share insights from a recent publication: ” Platelet transfusion induces transfusion-associated circulatory overload in rats with myocardial infarction.” (1). This study provides valuable understanding of transfusion-related complications in cardiac injury models, with a particular focus on monitoring cardiac stress and overload.

Key Highlight: Reliable detection of rat NT-proBNP in a TACO rat model in anemic Wistar rats with myocardial infarction using the Biomedica ELISA.

Accurate Detection of NT-proBNP in Rats – A Cardiac Safety Biomarker

A key tool in this research was the accurate quantification of NT-proBNP, a well-established biomarker for cardiac stress and heart failure. The researchers employed the Biomedica ELISA assay, renowned for its sensitivity and specificity, to measure NT-proBNP levels in rat plasma samples.

Rat NT-proBNP ELISA (cat. no. BI-1204R)

• CONVENIENT: Low sample volume (10 µL/well), kit control included
• HIGHLY SENSITIVE: Limit of detection (LOD) 21 pg/mL 
• QUICK RESULTS: Assay time 3.5 hours 
• TRUSTWORTHY RESULTS: Reference values provided, click here

 

The use of the Biomedica ELISA assay enabled precise detection of NT-proBNP levels, providing robust data on cardiac function and overload following platelet transfusion.

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Implications for Future Research

The successful application of the Biomedica Rat NT-proBNP ELISA assay in this context demonstrates its suitability for preclinical cardiac studies involving small animals. Researchers aiming to explore cardiac biomarkers in rat models can confidently utilize this assay to obtain reliable and meaningful data.

Interested in measuring NT-proBNP in your research?

• Explore the Biomedica ELISA assay and enhance your data accuracy today!
• Contact us (info@bmgrp.com  or +43 1 29107 54 ) ) for your evaluation kit!

 

Accurate Detection of NT-proBNP in Rats – A Cardiac Safety Biomarker

 

 

Related Products:

NT-proANP ELISA assay (cat. no. BI-20892)  

• CONVENIENT – 10 µl / well, serum or plasma, kit control included
• TRUSTED – widely cited as cardiovascular safety biomarker in rats
• MULTI-USE – for human and non-human samples (high cross-reactivity between species)

 

BIOMEDICA’s NT-proANP ELISA kit (BI-20892) has been independently validated through preclinical toxicology testing in rats. Click here to view citations

Cross-laboratory analytical validation of the cardiac biomarker NT-proANP in rat

Specifically, a cross-laboratory analytical validation of the cardiac biomarker NT-proANP in rats was conducted.

Briefly, the assay was evaluated by the Predictive Safety Testing Consortium’s Cardiac Hypertrophy Working Group (PSTC—CHWG) across five laboratories in a comprehensive analytical assessment for rat samples. The results demonstrated that the assay is robust and technically suitable for detecting NT-proANP serum levels in Sprague-Dawley rats.

 

Related Publications:

Evaluation of Cardiac Toxicity Biomarkers in Rats from Different Laboratories. Kim, K., Chini, N., Fairchild, D.G., Engle, S.K., Reagan, W.J., Summers, S.D., Mirsalis, J.C., 2016. Toxicol Pathol 44, 1072–1083. PMID: 27638646.

Serum Natriuretic Peptides as Differential Biomarkers Allowing for the Distinction between Physiologic and Pathologic Left Ventricular Hypertrophy . Dunn, M.E., Manfredi, T.G., Agostinucci, K., Engle, S.K., Powe J., King, N.M.P., Rodriguez, L.A, Gropp, K.E., Gallacher, M., Vetter, F.J., More, V., Shimpi, P., Serra, D., Colton, H.M., for The Cardiac Hypertrophy Working Group of the Predictive Safety Testing Consortium . 2017. Toxicol Pathol 45(2): 334-352. PMID: 27102652.

Natriuretic Peptides as Cardiovascular Safety Biomarkers in Rats: Comparison With Blood Pressure, Heart Rate, and Heart Weight . Engle, S.K., and Watson, D.E. 2016. Toxicol Sci 149: 458 – 472. PMID: 26609138.

2-Deoxy-d-Glucose (2-DG)-Induced Cardiac Toxicity in Rat: NT-proBNP and BNP as Potential Early Cardiac Safety Biomarkers. Terse PS, Joshi PS, Bordelon NR, Brys AM, Patton KM, Arndt TP, Sutula TP. Int J Toxicol. 2016 May;35(3):284-93. doi: 10.1177/1091581815624397. Epub 2016 Feb 2. PMID: 26838190; PMCID: PMC4864115.

also available: NT-proBNP ELISA (cat.no. SK-1204)

• Proficiency testing 
• For human serum and plasma samples  – saliva protocol
• Widely cited