BIOMEDICA Analytical Testing Service
BIOMEDICA provides custom analytical testing service for universities, biotechnology and pharmaceutical industries. Our skilled laboratory team conducts sample measurements for the quantification of biomarkers in various sample matrices. We deliver quality and reliable results tailored to your precise specifications. Our primary objective is to meet your specific goals! BIOMEDICA has a long track record in successfully conducting numerous research projects which have also resulted in publications in top journals.
BIOMEDICA Analytical Testing Service
ELISA ASSAYs for Biomarkers DEVELOPED AND MANUFACTURED BY BIOMEDICA
We measure biomarkers using our propriety ELISA kits for the quantification of analytes in serum, plasma, urine and cell culture supernatant. Check out our product portfolio
ELISA ASSAYS FOR BIOMARKERS FROM OTHER MANUFACTURERS
We measure biomarkers in any kind of ELISA assay of your choice for any kind of analyte
LUMINEX TECHNOLOGY MULTIPLEX ASSAYS
We measure biomarkers using the Luminex xMAP® (multiple analyte profiling) technology-based immunoassays that enable the simultaneous detection and quantification of multiple analytes.
NEXT-GENERATION SEQUENCING & RT-QPCR MICRORNA SERVICES
We provide a broad range of high-quality RNA services including RNA extraction, NGS and RT-qPCR and the custom analysis of microRNA signatures.
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Enzyme-Linked Immunosorbent Assay (ELISA). Tabatabaei MS, Ahmed M. Methods Mol Biol. 2022;2508:115-134. doi: 10.1007/978-1-0716-23763_10. PMID: 35737237.
Enzyme-linked immunosorbent assay (ELISA) is one of the most specific and straightforward assays for detecting biomolecules in research and clinics. With advances in analytical methods, ELISA assay has been constantly optimized to improve its sensitivity, and different types of ELISA are now available to detect various biomarkers. This chapter provides an overall summary of the basic principle of ELISA, discusses different components of ELISA assay, and clearly outline protocols for different types of ELISA assays, including direct, indirect, sandwich, competitive, and nanoparticle-based ELISA.
Circulating miRNAs in bone health and disease. Grillari J, Mäkitie RE, Kocijan R, Haschka J, Vázquez DC, Semmelrock E, Hackl M. Bone. 2021 Apr;145:115787. doi: 10.1016/j.bone.2020.115787. Epub 2020 Dec 8. PMID: 33301964.
microRNAs have evolved as important regulators of multiple biological pathways essential for bone homeostasis, and microRNA research has furthered our understanding of the mechanisms underlying bone health and disease. This knowledge, together with the finding that active or passive release of microRNAs from cells into the extracellular space enables minimal-invasive detection in biofluids (circulating miRNAs), motivated researchers to explore microRNAs as biomarkers in several pathologic conditions, including bone diseases. Thus, exploratory studies in cohorts representing different types of bone diseases have been performed. In this review, we first summarize important molecular basics of microRNA function and release and provide recommendations for best (pre-)analytical practices and documentation standards for circulating microRNA research required for generating high quality data and ensuring reproducibility of results. Secondly, we review how the genesis of bone-derived circulating microRNAs via release from osteoblasts and osteoclasts could contribute to the communication between these cells. Lastly, we summarize evidence from clinical research studies that have investigated the clinical utility of microRNAs as biomarkers in musculoskeletal disorders. While previous reviews have mainly focused on diagnosis of primary osteoporosis, we have also included studies exploring the utility of circulating microRNAs in monitoring anti-osteoporotic treatment and for diagnosis of other types of bone diseases, such as diabetic osteopathy, bone degradation in inflammatory diseases, and monogenetic bone diseases.