Biomedica Immunoassays

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ENDOSTATIN ELISA

Wide dynamic range.

Assay characteristics:

Cat.No.: BI-20742
Method: Sandwich ELISA, HRP/TMB, 12x8-well strips
Sample type:Urine, serum, plasma (EDTA, heparin, citrate)
Standard range:0-800 pmol/l (7 standards)
Standard points:0/25/50/100/200/400/800 pmol/l
Control:2 controls
Sample size:10-20 µl / well
Incubation time:3 h / 1 h / 30 min
Unit conversion:1 pg/ml = 0.05 pmol/l (MW: 20 kDa)

Sensitivity:
LOD: 4 pmol/l (0 pmol/l + 3 SD), LLOQ: 3 pmol/l

Precision:
Intra-assay (n=5) ≤ 6%, Inter-assay (n=16) ≤ 5%

Spike/Recovery:
The mean recovery of recombinant Endostatin in serum and citrate plasma is 95% and 91%, respectively.
The mean recovery of recombinant Endostatin in heparin plasma and EDTA plasma is 96% and 92%, respectively.  

Dilution linearity:
The mean dilution linearity of endogenous Endostatin in serum sampes is:
Mean for 1+29 dilution = 119%
Mean for 1+59 dilution = 120%
Mean for 1+119 dilution = 116%. 

Cross reactivity:
This assay recognizes endogenous and recombinant human Endostatin. There is no cross reactivitiy with other species. There is no cross reactivity with human recombinant Collagen Type XV (COL15).

Values from apparently healthy individuals:
Median (serum, n=59): 5.1 nmol/l.
It is recommended to establish the normal range for each laboratory.   

Principle of the assay: 



Manual ELISAs - easily adaptable for automation!