Biomedica Immunoassays

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Periostin ELISA, human

Extensively validated for clinical samples. - 7 human serum based standards and controls.

 Assay characteristics:

Cat.No.: BI-20433
Method: Sandwich ELISA, HRPO/TMB, 12x8-well strips
Sample type:Serum, plasma (EDTA, heparin, citrate)
Standard range:0-4000 pmol/l (7 serum based standards)
Standard points:0/125/250/500/1000/2000/4000 pmol/l
Control:2 serum based controls
Sample size:10 µl / sample
Incubation time:2 h / 2 h / 1 h / 30 min
Unit conversion:1 pg/ml = 0.011 pmol/l (MW: 91 kDa)

Sensitivity:
LOD: 20 pmol/l (0 pmol/l + 3 SD); LLOQ: 62.5 pmol/l

Precision:
Intra-assay: 2 samples of known concentrations were tested 5 times within 1 kit lot by 1 operator.
Inter-assay: 2 samples of known concentrations were tested 10 times within 3 different kit lots by 3 different operators.

Intra-assay (n=5) Sample 1 Sample 2
Mean (pmol/l) 249 2008
SD (pmol/l) 7.3 52
CV (%) 3 3
Inter-assay (n=10) Sample 1 Sample 2
Mean (pmol/l) 251 1996
SD (pmol/l) 11.2 111.5
CV (%) 4 6

Spike/Recovery:
The recovery of Periostin was evaluated by adding 2 known concentrations of human recombinant Periostin to different human sample matrices. 

Matrix Mean S/R [% ]
+500 pmol/l +2000 pmol/l
Serum (n=7) 106 95
EDTA plasma (n=8) 98 83
Heparin plasma (n=7) 92 85
Citrate plasma (n=8) 102 91

Dilution linearity:
Dilution linearity was assessed by serially diluting samples containing endogenous Periostin with assay buffer.

Matrix Mean R of dilution [% ]
1+1 1+3
Serum (n=12) 101 105
EDTA plasma (n=4) 99 115
Heparin plasma (n=4) 96 126
Citrate plasma (n=4) 95 122

Specificity:
This assay is optimized to detect all known splicing forms of human periostin. This assay recognizes recombinant and endogenous Periostin.

Cross-reactivity:
Due to the high sequence homology between human Periostin and Periostin of other species, the antibodies utilized in the assay may cross-react with mouse, rat, cynomolgous monkey, dog and cat Periostin.

Calibration:
This immunoassay is calibrated against recombinant human Periostin peptide.

Values from apparently healthy individuals:
Median serum (n=24): 864 pmol/l
Median EDTA plasma (n=20): 817 pmol/l
Median heparin plasma (n=20): 891 pmol/l
Median citrate plasma (n=24): 885 pmol/l
It is recommended to establish the normal range for each laboratory.

Principle of the assay: 



Manual ELISAs - easily adaptable for automation!