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NT-proANP ELISA

NT-proANP as Cardiovascular Safety Biomarker in Rats! CE registered - for IVD use in EU!

PRODUCT DETAILS

Assay characteristics:

Cat.No.: BI-20892
Method: Sandwich ELISA, HRP/TMB, 12x8-well strips
Sample type:Serum, plasma (EDTA, heparin), cell culture supernatant, urine
Standard range:0-10 nmol/l (6 serum based standards)
Standard points: 0/0.63/1.25/2.5/5/10 nmol/l
Control:1 serum based control
Sample size:10 µl / well
Incubation time:3 h / 30 min
Unit conversion:1 ng/ml = 0.079 nmol/l (MW: 12.7 kDa)

Sensitivity:
LOD: 0.05 nmol/l (0 pmol/l + 3 SD)

Precision:
Intra-assay (n=10) ≤ 2%, Inter-assay (n=5) ≤ 4%

Spike/Recovery:
The mean recovery of recombinant proANP (1-98) in human plasma samples (n=4) is 87%.  

Dilution linearity:
Dilution linearity of endogenous proANP (1-98) with assay buffer is:
Mean for 1+1 dilution = 90%
Mean for 1+3 dilution = 87%
Mean for 1+7 dilution = 79%

Cross reactivity:
The assay cross-reacts with rat or mouse samples.
proANP(1-30) <1%, proANP(31-67) <1%, proANP(79- 98) <1%, alpha ANP( 99-126)<1%, proBNP(8-29)<1%, proBNP(32-57) <1%, proCNP(1-19) <1%, proCNP(30-50) <1%, proCNP(51-97) <1%.

Values from apparently healthy individuals:
Median (plasma, n=53): 1.45 nmol/l. 
It is recommended to establish the normal range for each laboratory. 

Principle of the assay: 



Manual ELISAs - easily adaptable for automation! 

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INSTRUCTIONS FOR USE

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BI-20892 proANP ELISA IFU

Biomedica Analytical Service Quotation Form

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VALIDATION DATA

Click here for assay validation data (S/R, dilution linearity, precision, ...).

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ADDITIONAL INFORMATION

Atrial natriuretic peptide is synthesized in atrial myocytes and is stored in secretory granules as a 126 amino acid prohormone. The most important stimulus for the release of the hormone into circulation is stretch of the myocyte fibres. On release the prohormone is split into equimolar amounts of the highly biologically active proANP (99-126), also known as α-ANP, and the N-terminal part proANP (1-98). α-ANP is rapidly cleared from the circulation with a half-life of 3-4 minutes. proANP (1-98) has a much longer half-life (60-120 min) which leads to significantly higher concentrations in blood compared to α-ANP. Thus, circulating levels of proANP (1-98) are less sensitive to the pulsatile secretion of ANP and may better reflect chronic levels of ANP secretion than the rapidly fluctuating levels of α-ANP. proANP is discussed as valuable marker for e.g. sepsis (Increased plasma levels of NT-proANP and NT-proBNP as markers of cardiac dysfunction in septic patients. Hoffmann U. et al., Clin. Lab. 2005;51 (7-8):373-9), or risk stratification in heart failure (Neurohormonal risk stratification for sudden death and death owing to progressive heart failure in chronic heart failure. Berger R. et al, European Journal of Clinical Investigation, 2005, 35 (1), 24-31).

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REFERENCES, APPLICATIONS

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Reference for rat samples:

An initial characterization of N-terminal-proatrial natriuretic peptide in serum of Sprague Dawley rats. Colton HM et al, Toxicol Sci. 2011 Apr;120(2):262-268

Cross-laboratory analytical validation of the cardiac biomarker NT-proANP in rat.
Vinken P. et al, J Pharmacol Toxicol Methods. 2016 Jan-Feb;77:58-65.

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