|Method:||Sandwich ELISA, HRP/TMB, 12x8-well strips|
|Sample type:||Serum, plasma (EDTA, heparin, citrate)|
|Standard range:||0-10 µg/ml (6 serum based standards)|
|Standard points:||0/0.6/1.25/2.5/5/10 µg/ml|
|Control:||1 serum based control|
|Sample size:||20 µl / well|
|Incubation time:||1.5 h / 1.5 h / 30 min|
LOD: 0.05 µg/ml (0 µg/ml + 3 SD)
Intra-assay (n=3) ≤ 8%, Inter-assay (n=4) ≤ 7%
Values from apparently healthy individuals:
Median (blood donors, n=71): 1.0 µg/ml.
It is recommended to establish the normal range for each laboratory.
Principle of the assay:
Manual ELISAs - easily adaptable for automation!
INSTRUCTIONS FOR USE
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Oxidatively modified lipoproteins (oxLDLs) play an important role in the progression of atherosclerosis and coronary artery disease. Low-density lipoprotein (LDL), the main carrier of plasma cholesterol, consists of a hydrophobic core and a surface monolayer of polar lipids and Apolipoprotein-B (ApoB). Oxidative stress and the consequent formation of free radicals lead to the peroxidation of ApoB. Malondialdehyde (MDA) has been identified as one of the major lipid peroxidation products of LDL, thus playing an important role in the LDL oxidation.