Biomedica Immunoassays

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Borrelia IgG ELISA

Recombinant antigens for better reproducibility. - CE marked - for IVD use in EU.


Assay characteristics:

Cat.No.: BI-21032
Method: Sandwich ELISA, HRP/TMB, 12x8-well strips
Sample type:Serum, plasma, CSF
Controls:3 vials (negative, cut-off, positive)
Sample size:100 µl (diluted sample) / well
Incubation time:1 h / 30 min / 15 min

Serum: 100%, CSF: 88%

Serum: 96%, CSF: 88%

Intra-assay (n=8) ≤ 8%, Inter-assay (n=12) ≤ 12%

Principle of the assay:

Manual ELISAs - easily adaptable for automation! 

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Click link for: 

BI-21032 Borrelia IgG ELISA IFU

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Lyme borreliosis is a bacterial infection caused by the spirochaete Borrelia burgdorferi sensi stricto, afzellii, or garinii, and is characterised by a variety of clinical symptoms. It can be divided into 3 stages:

Stage 1, early dermatitis, Clinical: erythema migrans.

Stage 2, early disseminated infection, Clinical: lymphocytic meningoradiculitis (Bannwarth's syndrome), neuroborreliosis.

Stage 3, late disseminated infection, Clinical: chronic progressive encephalomyelitis, acrodermatitis chronica atrophicans (ACA), chronic arthritis.

To improve the diagnostic specifity, the Biomedica microwell strips are coated with the following recombinant antigens:
· p21 = OspC (outer surface protein C): B. burgdorferi sensu stricto (B31), B. garinii (20047)
· p18: B. afzelii (pKo)
· p100: B. afzelii (pKo)
· VIsE: fusion protein of different Borrelia genospecies 

Click here for MSDS
Click here for PRODUCT LEAFLET 

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