- Product Details
- Instructions For Use
- Validation Data
- Additional Information
- References, Applications
|Method:||Sandwich ELISA, HRP/TMB, 12x8-well strips|
|Sample type:||Serum, plasma (EDTA, heparin), cell culture supernatant, urine|
|Standard range:||0-10 nmol/l (6 serum based standards)|
|Standard points:||0/0.63/1.25/2.5/5/10 nmol/l|
|Control:||1 serum based control|
|Sample size:||10 µl / well|
|Incubation time:||3 h / 30 min|
|Unit conversion:||1 ng/ml = 0.079 nmol/l (MW: 12.7 kDa)|
LOD: 0.05 nmol/l (0 pmol/l + 3 SD)
Intra-assay (n=10) ≤ 2%, Inter-assay (n=5) ≤ 4%
The mean recovery of recombinant proANP (1-98) in human plasma samples (n=4) is 87%.
Dilution linearity of endogenous proANP (1-98) with assay buffer is:
Mean for 1+1 dilution = 90%
Mean for 1+3 dilution = 87%
Mean for 1+7 dilution = 79%
The assay cross-reacts with rat or mouse samples.
proANP(1-30) <1%, proANP(31-67) <1%, proANP(79- 98) <1%, alpha ANP( 99-126)<1%, proBNP(8-29)<1%, proBNP(32-57) <1%, proCNP(1-19) <1%, proCNP(30-50) <1%, proCNP(51-97) <1%.
Values from apparently healthy individuals:
Median (plasma, n=53): 1.45 nmol/l.
It is recommended to establish the normal range for each laboratory.
Principle of the assay:
Manual ELISAs - easily adaptable for automation!
INSTRUCTIONS FOR USE
Click link for:
Click here for assay validation data (S/R, dilution linearity, precision, ...).
Atrial natriuretic peptide is synthesized in atrial myocytes and is stored in secretory granules as a 126 amino acid prohormone. The most important stimulus for the release of the hormone into circulation is stretch of the myocyte fibres. On release the prohormone is split into equimolar amounts of the highly biologically active proANP (99-126), also known as α-ANP, and the N-terminal part proANP (1-98). α-ANP is rapidly cleared from the circulation with a half-life of 3-4 minutes. proANP (1-98) has a much longer half-life (60-120 min) which leads to significantly higher concentrations in blood compared to α-ANP. Thus, circulating levels of proANP (1-98) are less sensitive to the pulsatile secretion of ANP and may better reflect chronic levels of ANP secretion than the rapidly fluctuating levels of α-ANP. proANP is discussed as valuable marker for e.g. sepsis (Increased plasma levels of NT-proANP and NT-proBNP as markers of cardiac dysfunction in septic patients. Hoffmann U. et al., Clin. Lab. 2005;51 (7-8):373-9), or risk stratification in heart failure (Neurohormonal risk stratification for sudden death and death owing to progressive heart failure in chronic heart failure. Berger R. et al, European Journal of Clinical Investigation, 2005, 35 (1), 24-31).
Click here for FAQs
Click here for MSDS
Click here for PRODUCT LEAFLET
Click here for reference list.