- Product Details
- Instructions For Use
- Validation Data
- Additional Information
- References, Applications
|Method:||Sandwich ELISA, HRP/TMB, 12x8-well strips|
|Sample type:||Serum and EDTA plasma|
|Standard range:||0-640 pmol/l (5 serum based standards)|
|Standard points:||0/10/40/160/640 pmol/l|
|Control:||2 serum based controls|
|Sample size:||50 µl / well|
|Incubation time:||3 h / 30 min|
|Unit conversion:||1 pg/ml = 0.118 pmol/l (MW: 8.475 kDa)|
LOD: 3 pmol/l (0 pmol/l + 3 SD)
Intra-assay (n=3) ≤ 3%, Inter-assay (n=8) ≤ 7%
The mean recovery of recombinant NT-proBNP in serum samples (n=4) is 104%, in EDTA plasma samples (n=4) is 94%.
Dilution linearity for serum samples (n=3) is between 79-86% for endogenous NT-proBNP (1-76) and 112-119% for recombinant NT-proBNP (1-76).
Dilution linearity for EDTA plasma samples (n=2) is between 83-84% for endogenous NT-proBNP (1-76) and 83-86% for recombinant NT-proBNP (1-76).
The assay works with human samples only.
Values from apparently healthy individuals:
Median (serum, n=70): 5.8 pmol/l.
Median (EDTA plasma, n=28): 5.6 pmol/l.
It is recommended to establish the normal range for each laboratory.
Principle of the assay:
Manual ELISAs - easily adaptable for automation!
INSTRUCTIONS FOR USE
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SK-1204 NT-proBNP ELISA Validation Data (S/R, dilution linearity, precision, ...).
BNP is mainly expressed by ventricular myocardium in response to volume overload and increased filling pressure. BNP has a cleavable signal sequence. Mature BNP consists of 108 amino acids (proBNP or BNP-108), and undergoes cleavage resulting in physiologically active BNP-32 and additional C-terminal fragments (cf. http://www.uniprot.org/uniprot/P16860#PRO_0000001532), along with a physiologically inactive N-terminal peptide comprising amino acids 1-76, which is further degraded proteolytically.
BNP has a key role in cardiovascular homeostasis with biological actions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. A high concentration of BNP in the bloodstream is indicative of heart failure.
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