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Periostin mouse ELISA

Fully validated for pre-clinical research.

PRODUCT DETAILS

 Assay characteristics:

Cat.No.: BI-20433MS
Method: Sandwich ELISA, HRPO/TMB, 12x8-well strips
Sample type:Serum, plasma
Standard range:0-16 nmol/l
Standard points:0/0.5/1/2/4/8/16 nmol/l
Control:1 control
Sample size:5 µl / sample
Incubation time:3 h / 1 h / 30 min
Unit conversion:1 ng/ml = 0.011 nmol/l (MW: 90.1 kDa)

Sensitivity:
LOD: 0.003 nmol/l (0 nmol/l + 3 SD); LLOQ: 0.125 nmol/l

Precision:
Intra-assay: 2 samples of known concentrations were tested 5 times within 1 kit lot by 1 operator.
Inter-assay: 2 samples of known concentrations were tested 15 times within 2 different kit lots by 2 different operators.

Intra-assay (n=5) Sample 1 Sample 2
Mean (nmol/l) 1.0 7.8
SD (nmol/l) 0.06 0.14
CV (%) 6 2
Inter-assay (n=15) Sample 1 Sample 2
Mean (nmol/l) 1.0 7.9
SD (nmol/l) 0.06 0.22
CV (%) 6 3

Spike/Recovery:
The recovery of mouse Periostin was evaluated by adding 2 known concentrations of recombinant mouse Periostin to different mouse samples. 

Matrix Mean S/R [% ]
+1.6 nmol/l +8 nmol/l
Serum (n=4) 72 97
Plasma (n=4) 86 88

Dilution linearity:
Dilution linearity was assessed by serially diluting samples containing endogenous mouse Periostin with assay buffer.

Matrix Mean R of dilution [% ]
1+1 1+3
Serum (n=4) 116 113
Plasma (n=4) 128 130

Specificity:
This assay recognizes endogenous (natural) and recombinant mouse Periostin.

Calibration:
This immunoassay is calibrated against purified recombinant mouse Periostin peptide.

Periostin values from mouse serum samples:
Adult mice (n=28): 3.4 ± 0.9 nmol/l
4-week old mice (n=24): 8.1 ± 1.0 nmol/l
Female mice, 4-weeks old (n=13): 7.9 ± 1.2 nmol/l
Male mice, 4-weeks old (n=11): 8.4 ± 0.7 nmol/l
It is recommended to establish the normal range for each laboratory.

Principle of the assay: 



Manual ELISAs - easily adaptable for automation! 

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INSTRUCTIONS FOR USE

Click link for: 

BI-20433MS Periostin mouse ELISA IFU

Biomedica Analytical Service Quotation Form

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VALIDATION DATA

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BI-20433MS Periostin mouse ELISA Validation Data (S/R, dilution linearity, precision, ...).

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ADDITIONAL INFORMATION

Periostin (OSF-2) is secreted as a 91 kDa homodimeric soluble extracellular matrix protein expressed in collagen-rich fibrous connective tissues. Periostin is involved in osteoblast recruitment, attachment and spreading. It has been associated with the epithelial-mesenchymal transition in cancer and with the differentiation of mesenchyme in the developing heart. Periostin has functions in osteology, tissue repair, oncology, cardiovascular and respiratory diseases, and in various inflammatory settings. There are at least 7 isoforms of Periostin, caused by alternative splicing (http://www.uniprot.org/uniprot/Q15063).

Click link for: 

BI-20433MS Periostin mouse ELISA MSDS

 

Additional background information:

Alternative Names: POSTN, OSF-2, OSF2, PDLPN, periostin

Regarding the Periostin gene: Entrez/NCBI ID: 10631 Genecards: POSTN OMIM: 605740 Regarding Periostin's structure: PDB: 5WT7 Expression pattern and disease relevance: Protein Atlas: POSTN Uniport ID: Q15063 Pubmed references: NCBI 10631

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