Biomedica Immunoassays

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SCLEROSTIN ELISA

Most referenced ELISA!

Assay characteristics:

Cat.No.: BI-20492
Method: Sandwich ELISA, HRP/TMB, 12x8-well strips
Sample type:Serum, plasma (EDTA, heparin), urine protocol available
Standard range:0-240 pmol/l (6 serum based standards)
Standard points:0/15/30/60/120/240 pmol/l
Control:1 serum based control
Sample size:20 µl / well
Incubation time:Overnight / 1 h / 30 min
Unit conversion:1 pg/ml = 0.044 pmol/l (MW: 22.5 kDa)

Sensitivity:
LOD: 3.2 pmol/l (0 pmol/l + 3 SD); LLOQ: < 7.5 pmol/l

Precision:
Intra-assay (n=8) ≤ 7%, Inter-assay (n=6) ≤ 10%

Spike/Recovery:
The mean recovery of recombinant Sclerostin in human serum samples (n=6) is 94%.

Dilution linearity:
Endogenous Sclerostin:
The mean dilution linearity of endogenous Sclerostin in human serum samples (n=4) is 110% for dilution factors 1+1, 1+3, 1+7.

Recombinant Sclerostin:
The mean dilution linearity for recombinant Sclerostin in human serum samples (n=3) is 98% for dilution factors 1+1, 1+3.

Cross reactivity: 
The assay does not detect Noggin.
The assay does not detect Wise (SOSTDC1).

Species cross reactivity:
Rat, mouse: The assay does not detect rat or mouse Sclerostin.
Primates: The sequence homology of human Sclerostin to various primate species is >95%. It is likely that the assay can be used for these species. Internal validations have not been carried out. However, data from other laboratories show positive results.

Serum  values from apparently healthy individuals:
Median (serum, n=411): 24.14. pmol/l. 
It is recommended to establish the normal range for each laboratory. 

Principle of the assay: 


Manual ELISAs - easily adaptable for automation!