Biomedica Immunoassays

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ENDOSTATIN mouse/rat ELISA

Fully validated for pre-clinical research.

Assay characteristics:

Cat.No.: BI-20742MR
Method: Sandwich ELISA, HRP/TMB, 12x8-well strips
Sample type:Serum, plasma
Standard range:0-32 nmol/l
Standard points:0/1/2/4/8/16/32 pmol/l
Control:1 control
Sample size:5 µl / sample
Incubation time:2 h / 30 min
Unit conversion:1 ng/ml = 0.049 nmol/l (MW: 20.4 kDa)
or 1 nmol/l = 20.376 ng/ml

Sensitivity:
LOD: 0.24 nmol/l (0 pmol/l + 3 SD), LLOQ: 0.5 nmol/l

Precision:
Intra-assay (n=5) ≤ 9%, Inter-assay (n=15) ≤ 10%

Spike/Recovery:
The mean recovery of recombinant mouse Endostatin (spike 25 and 22 nmol/l) is:
Mouse serum (n=7): 95%
Mouse plasma (n=5): 91%
Rat serum (n=4): 97%

Dilution linearity:
Dilution linearity of recombinant and endogenous Endostatin was assessed by serially diluting samples with assay buffer.

Dilution:
      1+1
     1+3
    1+7
Mouse serum (n=6)108 / 101117 / 88114 / *
Mouse plasma (n=5) 101 / 107104 / 109103 / *
Rat serum (n=7)99 / 96n.a. / 89n.a. / *
Rat plasma (n=4)n.a. / 89n.a. / 80n.a. / *

* not detectable, n.a.: not analysed

Endostatin values from mouse/rat serum samples:
Mouse sera (C57BL6JOlaHsd) controls, 12 weeks (n=11): 6.7 ± 0.8 nmol/l
Wildtype mouse sera normal values, 12 weeks, female (n=10): 4.1 ± 0.6 nmol/l
Wildtype mouse sera normal values, 12 weeks, male (n=10): 5.4 ± 1.2 nmol/l
Wildtype norma rat sera, 12 weeks, male (n=8): 2.5 ± 0.4 nmol/l

Principle of the assay: 

 

Manual ELISAs - easily adaptable for automation!