Biomedica Immunoassays

4989 €

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osteomiR - microRNA Biomarkers

Novel fracture-risk assessment tool. Simple and standardized analysis.

Assay characteristics:

Cat.No.: TW-KT-011-OT
Method:RT-qPCR, relative quantification without standard curve
Kit content:miRCURY™ RNA extraction,
osteomiR panel, SYBR Green
Kit size:for 48 samples*
Sample type:Serum, plasma (EDTA)
Control:optional: reference serum available on request
Sample size:200 µl serum or plasma required
Incubation time:sample to data in 8 hours with 2 hours hands-on time

*): 11 microRNAs will be analysed for each sample resulting in 528 data points in total.

Sensitivity:
LOD: 10 copies/µl (6 replicates, 95% positive detection); LLOQ: 35 copies/µl

Precision:
Intra-assay (n=6) ≤ 20%, Inter-assay (n=3) ≤ 20%

Dilution linearity:
synthetic osteomiRs:
The mean dilution linearity of synthetic controls in human serum samples yields an R² of 0.99 over 6 logs (Cq-range from 20 to 35).

Species cross reactivity:
The sequence homology of osteomiRs in rats and mice is high, however, detection can only be guaranteed for 100% conserved sequences. The assay detects the following microRNAs in:
Rats: rno-miR-203a-3p, rno-miR-127-3p, rno-let-7b-5p, rno-miR-29b-3p, rno-miR-335, rno-miR23a-3p, rnomiR-451-5p
Mouse: mmu-miR-203-3p, mmu-miR-188-3p, mmu-miR-127-3p, mmu-let-7p-5p, mmu-miR-214-3p, mmu-miR-29b-3p, mmu-miR-335, mmu-miR-23a-3p, mmu-miR-451-5p

Upon request, customized-assays for complete coverage of rodent or primate osteomiRs can be designed.