Biomedica Immunoassays

GLYCOMICS - SIMPLIFIED GLYCOANALYSIS

Glycan Profiling Services based on LecChip™ (Lectin microarrays). New technology complementary to mass spectrometry and HPLC.

PRODUCT DETAILS

Application:

Detection of Changes in Glycosylation Pattern
Supporting Quality Control Activities,
(Batch-to-Batch Variation)
Comparibility Testing of Biosimilars
Monitoring of Biologicals (e.g. Protein Drugs)
Host Cell Characterization
Glyco-Biomarker Discovery
Characterization of Stem Cells & Differentiated Cells
Advanced Histochemistry (e.g. Marker Definition)
Production Process Optimization (IPC testing)

Sample type:Purified and crude samples, e.g. cell/tissue extracts,
living cells, cell culture supernatants, serum, urine
Sensitivity:High sensitivity using low sample volumes

Testing service:

Profiling technology:

Glycan profiling service in ISO/GMP certified lab

GlycoStation™ Reader 1200 for your laboratory and in-house testing

Using a glass slide based Lectin Microarray (LecChip™), the binding to 45 different natural Lectins with unique binding properties for carbohydrates is tested.



Detailed information on the technology can also be found on the Glycotechnica website.

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INSTRUCTIONS FOR USE

Contract Analysis:

Our Glycan Profiling Service is carried out in Vienna, Austria in an ISO/GMP certified lab. A comprehensive analysis and interpretation of the results will be provided.

Click link for:

Quotation Form GLYCAN PROFILING ANALYSIS SERVICE

Specification for GLYCAN PROFILING ANALYSIS SERVICE

Glycan Profiling PRODUCT LEAFLET

Or contact us to talk about the specifications of your project.

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ADDITIONAL INFORMATION

Did you know?

Glycoanalysis with the LecChipTM Technology is the quickest and easiest glycan structure profiling system. - For details click here.


This new technology enables simplified glycoanalysis for any complex glycans, even for mixtures, with high throughput, high sensitivity, and high accuracy without clipping glycans from carrier proteins.

• Complementary to mass spectometry and HPLC
• High sensitivity using low sample volumes
  (LOD: 100 pg/ml glycoprotein, 100 pM glycan, 103 cells)
• Differential analysis of purified and crude samples
  (e.g. cell/tissue extracts, living cells, cc supernatants, serum, urine, ...)
• Identification of N- and O-glycans
• Differentiation of isomers
• High throughput - simple and quick to use
• High versatility and applicability

Applications:

• Detection of Changes in Glycosylation Pattern
• Comparability Testing of Biosimilars
• Monitoring of Biologicals (e.g. Protein Drugs)
• Host Cell Characterization
• Glyco-Biomarker Discovery
• Characterization of Stem Cells & Differentiated Cells
• Advanced Histochemistry, eg. Marker Definition
• Production Process Optimization (IPC Testing)

Contract Analysis:

Our Glycan profiling service is carried out in Vienna, Austria in an ISO/GMP certified lab. We will provide a comprehensive analysis and interpretation of the results. For detailed information, please contact us to talk about the specifications of your project.

Quotation Form GLYCAN PROFILING ANALYSIS SERVICE

Specifcation for GLYCAN PROFILING ANALYSIS SERVICE

Glycan Profiling PRODUCT LEAFLET


Detailed information on the technology can also be found on the Glycotechnica website.

 

 

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REFERENCES, APPLICATIONS


Click here for APPLICATIONS/REFERENCES.


The use of lectin microarray for assessing glycosylation of therapeutic proteins.


Zhang L et al., MAbs. 2016, DOI: 10.1080/19420862.2016.1149662.

Authors info: Office of Biotechnology Products, Center for Drug Evaluation and Research, Food and Drug Administration


The laboratory determined the lectin binding patterns of a panel of fifteen therapeutic proteins, including eight monoclonal antibodies using commercial lectin chips.

“In particular, the lectin-based microarray was found to be highly sensitive to variations in the terminal carbohydrate structures such as galactose versus sialic acid epitopes. These data suggest that lectin microarray could be used for screening glycan patterns of therapeutic glycoproteins."

Click here for abstract.

Contact us to receive your free copy of the full publication.



Glycan analysis of therapeutic glycoproteins.

Zhang L et al., MAbs. 2016: 0, 1-11

Authors info: Office of Biotechnology Products, Center for Drug Evaluation and Research, Food and Drug Administration


The review provides an update of recent advances in glycan analysis, including the potential utility of lectin-based microarray for high throughput glycan profiling of therapeutic proteins

The laboratory tested over a dozen FDA-approved therapeutic glycoproteins (including nine mAbs) using commercial lectin chips (GlycoTechnica Ltd.). The authors state that “the derived glycan profiles (e.g., glycan variants and relative content) are generally consistent with the known glycosylation patterns of each product".

“Our data show promise for lectin microarrays in profiling glycan variants that are commonly present in therapeutic proteins". Compared to MSbased methods, which usually involve multiple sample processing steps, a lectin microarray directly works on intact glycoproteins with only minimal alteration to a testing sample (e.g., fluorescent labeling).

“The lectin microarray, when coupled with a sophisticated detection system, appears to provide a high throughput platform for rapid screening of glycan profiles of therapeutic proteins."

Click here for abstract.

Contact us to receive your free copy of the full publication.

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