Biomedica Immunoassays

575 €

Contact us!

NT-proANP ELISA

NT-proANP as Cardiovascular Safety Biomarker in Rats! CE registered - for IVD use in EU!

Assay characteristics:

Cat.No.: BI-20892
Method: Sandwich ELISA, HRP/TMB, 12x8-well strips
Sample type:Serum, plasma (EDTA, heparin), cell culture supernatant, urine
Standard range:0-10 nmol/l (6 serum based standards)
Standard points: 0/0.63/1.25/2.5/5/10 nmol/l
Control:1 serum based control
Sample size:10 µl / well
Incubation time:3 h / 30 min
Unit conversion:1 ng/ml = 0.079 nmol/l (MW: 12.7 kDa)

Sensitivity:
LOD: 0.05 nmol/l (0 pmol/l + 3 SD)

Precision:
Intra-assay (n=10) ≤ 2%, Inter-assay (n=5) ≤ 4%

Spike/Recovery:
The mean recovery of recombinant proANP (1-98) in human plasma samples (n=4) is 87%.  

Dilution linearity:
Dilution linearity of endogenous proANP (1-98) with assay buffer is:
Mean for 1+1 dilution = 90%
Mean for 1+3 dilution = 87%
Mean for 1+7 dilution = 79%

Cross reactivity:
The assay cross-reacts with rat or mouse samples.
proANP(1-30) <1%, proANP(31-67) <1%, proANP(79- 98) <1%, alpha ANP( 99-126)<1%, proBNP(8-29)<1%, proBNP(32-57) <1%, proCNP(1-19) <1%, proCNP(30-50) <1%, proCNP(51-97) <1%.

Values from apparently healthy individuals:
Median (plasma, n=53): 1.45 nmol/l. 
It is recommended to establish the normal range for each laboratory. 

Principle of the assay: 



Manual ELISAs - easily adaptable for automation!