Biomedica Immunoassays

650 €

Contact us!

NT-proCNP ELISA

High homology between species! - CE marked - for IVD use in EU!

Assay characteristics:

Cat.No.: BI-20872
Method: Sandwich ELISA, HRP/TMB, 12x8-well strips
Sample type:Serum, plasma
Standard range:0-40 pmol/l (6 ready-to-use standards)
Standard points:0/2.5/5/10/20/40 pmol/l
Control:1 ready-to-use control
Sample size:50 µl / well
Incubation time:4 h / 30 min 
Unit conversion:1 pg/ml = 0.151 pmol/l (MW: 6.6 kDa)

Sensitivity:
LOD: 0.2 pmol/l (0 pmol/l + 3 SD)

Precision:
Intra-assay (n=5) ≤ 6%, Inter-assay (n=30) ≤ 1%

Cross reactivity:
Homology to other species for the epitopes used in this assay:
Epitope 1: rat (88%), sheep (94%), pig (94%), mouse (88%), bovine (88%)
Epitope 2: rat (95%), sheep (90%), pig (95%), mouse (95%), bovine (90%)

Values from apparently healthy individuals:
Median (serum, n=42) = 2.7 pmol/l 
It is recommended to establish the normal range for each laboratory.

Principle of the assay: 



Manual ELISAs - easily adaptable for automation!