Biomedica Immunoassays

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ENDOTHELIN ELISA

Direct measurement! - CE marked - for IVD use in EU!

Assay characteristics:

Cat.No.: BI-20052
Method: Sandwich ELISA, HRP/TMB, 12x8-well strips
Sample type:Serum, EDTA plasma
Standard range:0-10 fmol/ml (6 serum based standards) 
Standard points:0/0.625/1.25/2.5/5/10 fmol/ml
Controls:2 serum based controls
Sample size:50 µl / well
Incubation time:overnight / 1 h / 30 min
Unit conversion:1 pg/ml = 0.4 fmol/ml (MW: 2.49 kDa)

Sensitivity:
LOD: 0.02 fmol/ml (0 fmol/ml + 3 SD)

Precision:
Intra-assay (n=18) ≤ 4%, Inter-assay (n=24) ≤ 6%

Spike/Recovery:
The mean recovery of recombinant Endothelin (spike = 1 fmol/ml) is 96%.
The mean recovery of recombinant Endothelin (spike = 5 fmol/ml) is 86%.

Cross reactivity:
ET(1-21): 100%, ET2 (1-21): 100%, ET3 (1-21): <5%, Big Endothelin (1-38): <1%, Big Endothelin ( 22-38): <1%.
In normal human plasma samples ET-2 is estimated to be present at less than 20% of the ET-1 level. ET-3 is estimated to be present at 50% of the ET-1 level.

Animal sera: horse, cat, pig 100%; dog 66% and rat 79%, Mouse sera can not be measured in this ELISA.

Values from apparently healthy individuals:
Median (n=70): 0.26 fmol/ml.
It is recommended to establish the normal range for each laboratory.

Principle of the assay: 



Manual ELISAs - easily adaptable for automation!