Biomedica Immunoassays

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BNP FRAGMENT EIA

Direct measurement!

Assay characteristics:

Cat.No.: BI-20852W
Method: Competitive EIA, HRP/TMB, 12x8-well strips
Sample type:Serum, plasma (EDTA, heparin, citrate)
Standard range:0-6400 pmol/l (7 serum based standards)
Standard points: 0/200/400/800/1600/3200/6400 pmol/l
Control:1 serum based control
Sample size:30 µl / well
Incubation time:Overnight (18-25h) / 20 min
Unit conversion:1 pg/ml = 0.118 pmol/l (MW: 8.475 kDa)

Sensitivity:
LOD: 171 pmol/l at 95% B/B0

Precision:
Intra-assay (n=3) ≤ 8%, Inter-assay (n=8) ≤ 7%

Spike/Recovery:
The mean recovery of recombinant BNP Fragment in serum (n=4) is 100%. 
The mean recovery of recombinant BNP Fragment in citrate plasma (n=8) is 108%.

Dilution linearity:
The mean dilution linearity of recombinant BNP Fragment in human serum samples (n=7) is 118% for dilution factors 1+1 and 1+3.

Cross reactivity:
The assay does not cross react with rat, mouse, dog, or cat samples. 

Values from apparently healthy individuals:
Median (serum, n=76): 392 pmol/l.  
It is recommended to establish the normal range for each laboratory.

Principle of the assay: 



Manual ELISAs - easily adaptable for automation!