Biomedica Immunoassays

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BIG ENDOTHELIN ELISA

Direct measurement! - CE marked - for IVD use in EU!

Assay characteristics:

Cat.No.: BI-20082H
Method: Sandwich ELISA, HRP/TMB, 12x8-well strips
Sample type:Serum, plasma (EDTA, heparin, citrate)
Standard range:0-3 pmol/ml (6 serum based standards)
Standard points:0/0.1/0.2/0.4/1/3 pmol/l
Control:1 serum based control
Sample size:50 µl / well
Incubation time:4 h / 1 h / 30 min
Unit conversion:1 pg/ml = 0.2335 fmol/ml (MW: 4.283 kDa)

Sensitivity:
LOD: 0.02 pmol/l (0 pmol/l + 3 SD)

Precision:
Intra-assay (n=5) ≤ 5%, Inter-assay (n=10) ≤ 4%

Spike/Recovery:
The mean recovery of recombinant Big ET (spike 1 pmol/l; 2 pmol/l) is: 
Serum (n=14): 98%; 105% 
EDTA plasma (n=3) : 101%; 105%
Heparin plasma (n=3): 98%; 102%
Citrate plasma (n=3): 99%; 105% 

Dilution linearity:
The mean dilution linearity of endogenous Big ET with a low measuring human serum is:
For a 1+1 dilution = 90%
For a 1+3 dilution = 96%
For a 1+7 dilution = 98%

Cross reactivity:
ET1/2/3 (1-21): <1%, ET2 (1-37): <1%, ET1/2 (1-38): <1%, porcine BigET (1-39): 21%, BigET1/2 (22-38): <1%, BigET2 (22-37): <1%, rat BigET1 (1-39): 10%, Sarafotoxin: <1%

Values from apparently healthy individuals:
Median (serum, n=41): 0.09 pmol/l
It is recommended to establish the normal range for each laboratory.

Principle of the assay: 



Manual ELISAs - easily adaptable for automation!