Biomedica Immunoassays

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FREE soluble RANKL ELISA

HIGH SENSITIVITY - Measurable concentrations of free soluble uncomplexed RANKL in healthy subjects.

Assay characteristics:

Cat.No.: BI-20462
Method: Sandwich ELISA, HRP/TMB, 12x8-well strips
Sample type:Serum, heparin plasma
Standard range:0-2 pmol/l (7 serum based standards)
Standard points: 0/0.0625/0.125/0.25/0.5/1/2 pmol/l
Controls:2 serum based controls
Sample size:150 µl / well
Incubation time:2 h / overnight / 1 h / 30 min
Unit conversion:1 pg/ml = 0.05 pmol/l (MW: 20 kDa, monomer)

Sensitivity:
LOD: 0.01 pmol/l (0 pmol/l + 3 SD); LLOQ: 0.008 pmol/l

Precision:
Intra-assay (n=5) ≤ 5%, Inter-assay (n=12) ≤ 3%

Spike/Recovery:
The mean recovery of endogenous free sRANKL (spike: 0.15 pmol/l; 0.34 pmol/l) is: 
Serum: 89%; 85%
Heparin plasma: 100%; 98%

Dilution linearity:
Dilution linearity of endogenous free sRANKL with a low measuring serum as dilution medium is:    
Serum: 1+1 dilution = 112%, 1+3 dilution = 121%
Heparin plasma: 1+1 dilution = 121%

Cross reactivity: 
The sequence homology to various primates is >95%. It is likely that the assay can be used for these species. Internal validations have not been carried out.

Values from apparently healthy individuals:
Median (serum, n=32): 0.14 pmol/l. 
It is recommended to establish the normal range for each laboratory. 

Principle of the assay: 



Manual ELISAs  easily adaptable for automation!