FGF23 (C-terminal) ELISA
- Product Details
- Instructions For Use
- Validation Data
- Additional Information
- References, Applications
|Method:||Sandwich ELISA, HRP/TMB, 12x8-well strips|
|Sample type:||Serum, plasma (EDTA, heparin, citrate)|
|Standard range:||0-20 pmol/l (7 serum based standards)|
|Standard points:||0/0.2/0.6/1.8/5/10/20 pmol/l|
|Control:||2 serum based controls|
|Sample size:||50 µl / well|
|Incubation time:||20-24 h / 1 h / 30 min|
|Unit conversion:||1 pg/ml = 0.133 pmol/l (MW: 7.52 kDa)|
LOD: 0.08 pmol/l (0 pmol/l + 3 SD); LLOQ: 0.1 pmol/l
Intra-assay: 2 samples of known concentrations were tested 6 times within 1 kit lot by 1 operator.
Inter-assay: 2 samples of known concentrations were tested 10 times within 2 different kit lots by 4 different operators.
|Intra-assay (n=6)||Sample 1||Sample 2|
|Inter-assay (n=10)||Sample 1||Sample 2|
The recovery of FGF23 was evaluated by adding 2 concentrations of human recombinant C-terminal FGF23 (5 + 10 pmol/l) to different human sample matrices.
|Matrix||Mean S/R [% ]|
|+5 pmol/l||+10 pmol/l|
|EDTA plasma (n=7)||97||94|
|Heparin plasma (n=8)||101||92|
|Citrate plasma (n=7)||100||90|
Dilution linearity was assessed by serially diluting samples containing endogenous FGF23 with assay buffer.
|Matrix||Mean R of dilution steps [%]|
|EDTA plasma (n=4)||103||103||106|
|Heparin plasma (n=10)||107||106||104|
|Citrate plasma (n=5)||102||106||101|
The assay measures both intact FGF23 and C-terminal FGF23. The assay recognizes endogenous (natural) and recombinant human FGF23.
This immunoassay is calibrated against recombinant human FGF23 (C-terminal) peptide.
Values from apparently healthy individuals:
Median serum (n=35): 0.8 pmol/l
Median EDTA plasma (n=22): 1.3 pmol/l
Median heparin plasma (n=22): 1.2 pmol/l
Median citrate plasma (n=30): 1.4 pmol/l
It is recommended to establish the normal range for each laboratory.
Principle of the assay:
Manual ELISAs - easily adaptable for automation!