Biomedica Immunoassays

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DKK-1 ELISA

Direct measurement! - No sample pre-dilution! - CE registered!

Assay characteristics:

Cat.No.: BI-20413
Method: Sandwich ELISA, HRP/TMB, 12x8-well strips
Sample type:Serum
Standard range:0-160 pmol/l (6 serum based standards)
Standard points:0/10/20/40/80/160 pmol/l
Control:1 serum based control
Sample size:20 µl / well
Incubation time:2 h / 1 h / 30 min
Unit conversion:1 pg/ml = 0.039 pmol/l (MW: 25.8 kDa)

Specificity:
oligeomeric forms of natural and recombinant DKK-1.

Sensitivity:
LOD: 1.7 pmol/l (0 pmol/l + 3 SD)

Precision:
Intra-assay (n=5) ≤ 3%, Inter-assay (n=9) ≤ 3%

Spike/Recovery:
The mean recovery of recombinant DKK-1 in human serum samples (n=8) is 92% (spike: 40 pmol/l) and 83% (spike: 80 pmol/l). 

Dilution linearity:
Average recovery of expected DKK-1 after a 1+1; 1+3; 1+7 dilution in serum (n=4) is 109%; 104%; 100%

Cross reactivity: 
Human samples only, no cross reactivity with other species. No cross reactivity or interference with recombinant human DKK-4, Kremen-1, Kremen-2, or LRP-6 was observed.

Values from apparently healthy individuals:
Median (serum, n=51) = 34 pmol/l  
It is recommended to establish the normal range for each laboratory. 

Principle of the assay: 
 

Manual ELISAs - easily adaptable for automation!