- Product Details
- Instructions For Use
- Validation Data
- Additional Information
- References, Applications
|Method:||Sandwich ELISA, HRP/TMB, 12x8-well strips|
|Sample type:||Serum, plasma (EDTA, heparin, citrate)|
|Standard range:||0-20 pmol/l (6 serum based standards)|
|Standard points:||0/1.25/2.5/5/10/20 pmol/l|
|Control:||1 serum based control|
|Sample size:||20 µl / well|
|Incubation time:||4 h / 1 h / 30 min|
|Unit conversion:||1 pg/ml = 0.05 pmol/l (MW: 19.9 kDa)|
LOD: 0.07 pmol/l (0 pmol/l + 3 SD); LLOQ: < 0.08 pmol/l
Intra-assay (n=5) ≤ 3%, Inter-assay (n=12) ≤ 5%
The mean recovery of recombinant OPG in serum and EDTA plasma is between 93-108%.
The mean recovery of recombinant OPG in heparin - and citrate plasma is between 82-109%.
The dilution linearity of endogenous OPG in serum samples (n=3) is between 79-102%.
The dilution linearity of recombinant OPG in serum samples (n=8) is between 80-116%.
The assay does not cross react with rat or mouse samples.
Values from apparently healthy individuals:
Median (serum, n=60): 2.7 pmol/l.
It is recommended to establish the normal range for each laboratory.
Principle of the assay:
Manual ELISAs - easily adaptable for automation!
INSTRUCTIONS FOR USE
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Click here for assay validation data (S/R, dilution linearity, precision, ...).
Osteoprotegerin (OPG) or osteoclast inhibitory factor (OCIF) is a glycoprotein of the TNF receptor superfamily 11b (gene name TNFRSF11B, http://www.uniprot.org/uniprot/O00300). OPG is synthesized as a monomer of 380 amino acids and is assembled as a homodimer within the cell, and then secreted mainly as a disulfide-linked homodimer into the extracellular compartment. OPG is produced by many different tissues and cell types including osteoblasts. OPG is a negative regulator of bone resorption by acting as decoy receptor for RANKL, thus neutralizing its function in osteoclastogenesis. This glycoprotein is also involved in the regulation of vascular calcification.
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