Biomedica Immunoassays

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ENDOSTATIN mouse/rat ELISA

Fully validated for pre-clinical research.

PRODUCT DETAILS

Assay characteristics:

Cat.No.: BI-20742MR
Method: Sandwich ELISA, HRP/TMB, 12x8-well strips
Sample type:Serum, plasma
Standard range:0-32 nmol/l
Standard points:0/1/2/4/8/16/32 pmol/l
Control:1 control
Sample size:5 µl / sample
Incubation time:2 h / 30 min
Unit conversion:1 ng/ml = 0.049 nmol/l (MW: 20.4 kDa)
or 1 nmol/l = 20.376 ng/ml

Sensitivity:
LOD: 0.24 nmol/l (0 pmol/l + 3 SD), LLOQ: 0.5 nmol/l

Precision:
Intra-assay (n=5) ≤ 9%, Inter-assay (n=15) ≤ 10%

Spike/Recovery:
The mean recovery of recombinant mouse Endostatin (spike 25 and 22 nmol/l) is:
Mouse serum (n=7): 95%
Mouse plasma (n=5): 91%
Rat serum (n=4): 97%

Dilution linearity:
Dilution linearity of recombinant and endogenous Endostatin was assessed by serially diluting samples with assay buffer.

Dilution:
      1+1
     1+3
    1+7
Mouse serum (n=6)108 / 101117 / 88114 / *
Mouse plasma (n=5) 101 / 107104 / 109103 / *
Rat serum (n=7)99 / 96n.a. / 89n.a. / *
Rat plasma (n=4)n.a. / 89n.a. / 80n.a. / *

* not detectable, n.a.: not analysed

Endostatin values from mouse/rat serum samples:
Mouse sera (C57BL6JOlaHsd) controls, 12 weeks (n=11): 6.7 ± 0.8 nmol/l
Wildtype mouse sera normal values, 12 weeks, female (n=10): 4.1 ± 0.6 nmol/l
Wildtype mouse sera normal values, 12 weeks, male (n=10): 5.4 ± 1.2 nmol/l
Wildtype norma rat sera, 12 weeks, male (n=8): 2.5 ± 0.4 nmol/l

Principle of the assay: 

 

Manual ELISAs - easily adaptable for automation!

 

 

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INSTRUCTIONS FOR USE

Click following link for the instruction for use: 

BI-20742MR - Endostatin ms/rt ELISA IFU

Biomedica Analytical Service Quotation Form

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VALIDATION DATA

Click following link for assay validation data (S/R, dilution linearity, precision, ...):

BI-20742MR - Endostatin ms/rt ELISA Validation Data

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ADDITIONAL INFORMATION

Endostatin, a 20-kDa C-terminal proteolytic fragment of collagen XVIII, is an endogenous angiogenesis inhibitor localized in the vascular basement membrane in various organs (http://www.uniprot.org/uniprot/P39060). The biological functions of the endostatin-network involve SPARC, thrombospondin-1, glycosaminoglycans, collagens, and integrins.

In animal studies, renal Endostatin expression preceded deteriorating kidney function and induced renal fibrosis in aging mice. In humans, Endostatin is expressed during the progression of renal fibrosis in tubular cells of injured tissue .In renal micro-vascular disease, observed in late stages of patients with chronic kidney disease, increased endostatin levels are possibly the consequence of enhanced extracellular matrix degradation. Thus endostatin may become an important marker for progressive microvascular renal disease in patients with chronic kidney disease. Endostatin levels in blood are also likely to increase in patients with other microvascular tissue injuries, including atherosclerosis, myocardial- and brain ischemia. In ischemic stroke patients, high endostatin plasma levels predict a worse long-term clinical outcome. In a cohort of critically ill patients, plasma endostatin improved AKI prediction based on clinical risk factors. Endostatin has evolved as a molecular target and is currently under investigation in clinical trials.

Click link for: BI-20742MR - Endostatin ms/rt ELISA MSDS

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