BNP Fragment EIA
- Product Details
- Instructions For Use
- Validation Data
- Additional Information
- References, Applications
|Method:||Competitive EIA, HRP/TMB, 12x8-well strips|
|Sample type:||Serum, plasma (EDTA, heparin, citrate)|
|Standard range:||0-6400 pmol/l (7 serum based standards)|
|Standard points:||0/200/400/800/1600/3200/6400 pmol/l|
|Control:||1 serum based control|
|Sample size:||30 µl / well|
|Incubation time:||Overnight (18-25h) / 20 min|
|Unit conversion:||1 pg/ml = 0.118 pmol/l (MW: 8.475 kDa)|
LOD: 171 pmol/l at 95% B/B0
Intra-assay (n=3) ≤ 8%, Inter-assay (n=8) ≤ 7%
The mean recovery of recombinant BNP Fragment in serum (n=4) is 100%.
The mean recovery of recombinant BNP Fragment in citrate plasma (n=8) is 108%.
The mean dilution linearity of recombinant BNP Fragment in human serum samples (n=7) is 118% for dilution factors 1+1 and 1+3.
The assay does not cross react with rat, mouse, dog, or cat samples.
Values from apparently healthy individuals:
Median (serum, n=76): 392 pmol/l.
It is recommended to establish the normal range for each laboratory.
Principle of the assay:
Manual ELISAs - easily adaptable for automation!
INSTRUCTIONS FOR USE
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Click here for assay validation data (S/R, dilution linearity, precision, ...).
The natriuretic peptides are members of a family of structurally similar but genetically distinct peptide hormones, consisting of atrial-, brain-, and C-type (ANP, BNP, and CNP, respectively). ANP and BNP preferentially bind to a membrane-bound guanylyl cyclase (GC) receptor called GC-A or NPR1, whereas CNP is the physiological ligand for GC-B (NPR2). The natriuretic peptides play an important role in the regulation of cardiovascular and renal homeostasis and in the regulation of fatty acid metabolism and body weight.
BNP is mainly expressed by ventricular myocardium in response to volume overload and increased filling pressure. BNP has a cleavable signal sequence. Mature BNP consists of 108 amino acids (proBNP or BNP-108), and undergoes cleavage resulting in physiologically active BNP-32 and additional C-terminal fragments (cf. www.uniprot.org/uniprot/P16860, along with a physiologically inactive N-terminal peptide comprising amino acids 1-76, which is further degraded proteolytically. BNP fragments in the circulation are therefore very heterogenous.
BNP has a key role in cardiovascular homeostasis with biological actions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. A high concentration of BNP in the bloodstream is indicative of heart failure.
The discovery of natriuretic peptides identified an endocrine system that contributes to diuresis and vascular tone. The biology, biochemistry, and the pathophysiological role of natriuretic peptides are described in several reviews.
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