Biomedica Immunoassays

550 €

Contact us!


Direct measurement! - CE marked - for IVD use in EU!


Assay characteristics:

Cat.No.: BI-20082H
Method: Sandwich ELISA, HRP/TMB, 12x8-well strips
Sample type:Serum, plasma (EDTA, heparin, citrate)
Standard range:0-3 pmol/ml (6 serum based standards)
Standard points:0/0.1/0.2/0.4/1/3 pmol/l
Control:1 serum based control
Sample size:50 µl / well
Incubation time:4 h / 1 h / 30 min
Unit conversion:1 pg/ml = 0.2335 fmol/ml (MW: 4.283 kDa)

LOD: 0.02 pmol/l (0 pmol/l + 3 SD)

Intra-assay (n=5) ≤ 5%, Inter-assay (n=10) ≤ 4%

The mean recovery of recombinant Big ET (spike 1 pmol/l; 2 pmol/l) is: 
Serum (n=14): 98%; 105% 
EDTA plasma (n=3) : 101%; 105%
Heparin plasma (n=3): 98%; 102%
Citrate plasma (n=3): 99%; 105% 

Dilution linearity:
The mean dilution linearity of endogenous Big ET with a low measuring human serum is:
For a 1+1 dilution = 90%
For a 1+3 dilution = 96%
For a 1+7 dilution = 98%

Cross reactivity:
ET1/2/3 (1-21): <1%, ET2 (1-37): <1%, ET1/2 (1-38): <1%, porcine BigET (1-39): 21%, BigET1/2 (22-38): <1%, BigET2 (22-37): <1%, rat BigET1 (1-39): 10%, Sarafotoxin: <1%

Values from apparently healthy individuals:
Median (serum, n=41): 0.09 pmol/l
It is recommended to establish the normal range for each laboratory.

Principle of the assay: 

Manual ELISAs - easily adaptable for automation! 

Back to top


Click link for: 

BI-20082H Big Endothelin ELISA IFU

Biomedica Analytical Service Quotation Form

Back to top


Big Endothelin-1 (BigET) is a peptide of 38 amino acids and is the precursor of Endothelin-1 (ET), represented by amino acids 1-21 ( ET is a potent vasoconstrictor and is produced by vascular endothelial cells. Accordingly it has a wide tissue distribution ( The cleavage of BigET by Endothelin Converting Enzyme (ECE) leads to ET and to a C-terminal fragment. Both BigET and ET are strong independent predictors of survival in patients with congestive heart failure, and identify a population with a very high risk mortality. The half-life of ET (1-21) in plasma is less than one minute, whereas clearance of BigET is much slower. BigET can therefore be determined more easily.

Click here for MSDS
Click here for PRODUCT LEAFLET 

Back to top


Click here for reference list.

Back to top