Biomedica Immunoassays

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Periostin ELISA, human

Extensively validated for clinical samples. - 7 human serum based standards and controls.

PRODUCT DETAILS

 Assay characteristics:

Cat.No.: BI-20433
Method: Sandwich ELISA, HRPO/TMB, 12x8-well strips
Sample type:Serum, plasma (EDTA, heparin, citrate)
Standard range:0-4000 pmol/l (7 serum based standards)
Standard points:0/125/250/500/1000/2000/4000 pmol/l
Control:2 serum based controls
Sample size:10 µl / sample
Incubation time:2 h / 2 h / 1 h / 30 min
Unit conversion:1 pg/ml = 0.011 pmol/l (MW: 91 kDa)

Sensitivity:
LOD: 20 pmol/l (0 pmol/l + 3 SD); LLOQ: 62.5 pmol/l

Precision:
Intra-assay: 2 samples of known concentrations were tested 5 times within 1 kit lot by 1 operator.
Inter-assay: 2 samples of known concentrations were tested 10 times within 3 different kit lots by 3 different operators.

Intra-assay (n=5) Sample 1 Sample 2
Mean (pmol/l) 249 2008
SD (pmol/l) 7.3 52
CV (%) 3 3
Inter-assay (n=10) Sample 1 Sample 2
Mean (pmol/l) 251 1996
SD (pmol/l) 11.2 111.5
CV (%) 4 6

Spike/Recovery:
The recovery of Periostin was evaluated by adding 2 known concentrations of human recombinant Periostin to different human sample matrices. 

Matrix Mean S/R [% ]
+500 pmol/l +2000 pmol/l
Serum (n=7) 106 95
EDTA plasma (n=8) 98 83
Heparin plasma (n=7) 92 85
Citrate plasma (n=8) 102 91

Dilution linearity:
Dilution linearity was assessed by serially diluting samples containing endogenous Periostin with assay buffer.

Matrix Mean R of dilution [% ]
1+1 1+3
Serum (n=12) 101 105
EDTA plasma (n=4) 99 115
Heparin plasma (n=4) 96 126
Citrate plasma (n=4) 95 122

Specificity:
This assay is optimized to detect all known splicing forms of human periostin. This assay recognizes recombinant and endogenous Periostin.

Cross-reactivity:
Due to the high sequence homology between human Periostin and Periostin of other species, the antibodies utilized in the assay may cross-react with mouse, rat, cynomolgous monkey, dog and cat Periostin.

Calibration:
This immunoassay is calibrated against recombinant human Periostin peptide.

Values from apparently healthy individuals:
Median serum (n=24): 864 pmol/l
Median EDTA plasma (n=20): 817 pmol/l
Median heparin plasma (n=20): 891 pmol/l
Median citrate plasma (n=24): 885 pmol/l
It is recommended to establish the normal range for each laboratory.

Principle of the assay: 



Manual ELISAs - easily adaptable for automation! 

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INSTRUCTIONS FOR USE

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BI-20433 Periostin ELISA IFU

Biomedica Analytical Service Quotation Form

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VALIDATION DATA

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ADDITIONAL INFORMATION

Periostin (OSF-2) is secreted as a 91 kDa homodimeric soluble extracellular matrix protein expressed in collagen-rich fibrous connective tissues. Periostin is involved in osteoblast recruitment, attachment and spreading. It has been associated with the epithelial-mesenchymal transition in cancer and with the differentiation of mesenchyme in the developing heart. Periostin has functions in osteology, tissue repair, oncology, cardiovascular and respiratory diseases, and in various inflammatory settings. There are at least 7 isoforms of Periostin, caused by alternative splicing (http://www.uniprot.org/uniprot/Q15063).

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REFERENCES, APPLICATIONS

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