- Product Details
- Instructions For Use
- Validation Data
- Additional Information
- References, Applications
|Method:||Sandwich ELISA, HRP/TMB, 12x8-well strips|
|Standard range:||0-160 pmol/l (6 serum based standards)|
|Standard points:||0/10/20/40/80/160 pmol/l|
|Control:||1 serum based control|
|Sample size:||20 µl / well|
|Incubation time:||2 h / 1 h / 30 min|
|Unit conversion:||1 pg/ml = 0.039 pmol/l (MW: 25.8 kDa)|
oligeomeric forms of natural and recombinant DKK-1.
LOD: 1.7 pmol/l (0 pmol/l + 3 SD)
Intra-assay (n=5) ≤ 3%, Inter-assay (n=9) ≤ 3%
The mean recovery of recombinant DKK-1 in human serum samples (n=8) is 92% (spike: 40 pmol/l) and 83% (spike: 80 pmol/l).
Average recovery of expected DKK-1 after a 1+1; 1+3; 1+7 dilution in serum (n=4) is 109%; 104%; 100%
Human samples only, no cross reactivity with other species. No cross reactivity or interference with recombinant human DKK-4, Kremen-1, Kremen-2, or LRP-6 was observed.
Values from apparently healthy individuals:
Median (serum, n=51) = 34 pmol/l
It is recommended to establish the normal range for each laboratory.
Principle of the assay:
Manual ELISAs - easily adaptable for automation!
INSTRUCTIONS FOR USE
Click links for:
Click here for assay validation data (S/R, dilution linearity, precision, ...).
Dickkopf-1 (DKK-1) is a 28.67 Da secreted protein that acts as soluble inhibitor of the Wnt signalling pathway.
This pathway contains lipid-modified glycoproteins that activate cell surface receptor-mediated signal transduction to regulate cell activities like: cell fate, proliferation, migration, polarity, and gene expression.
DKK-1 regulates developmental processes of all kinds. Thus, DKK-1 is also involved in the regulation of bone metabolism as it effects osteoblast differentiation and in regulation of tumourgenetic activity.
Click here for reference list.